Applications

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Our bioassay system can be used for real-time monitoring of a wide variety of tests, including protein-protein, protein-drug, protein-peptide, cell-based (Figure 1), biomarker screening (Figures 2 and 3) and many others. Since our technology does not require the light to pass through the sample, our system operates with outstanding performance in a wide variety of backgrounds including (but not limited to): serum, plasma, cell culture media, food products (such as rinse water or milk) and environmental samples.

Contact us for detailed application notes.

  Some example applications include:

Cell-based Assays Example: Real-time Cellular Response to Drug/Small Molecule Interactions

Figure 1.  Analysis of various concentrations of staphylococcal enterotoxin B detected in PBS.

Figure 1. Measured cell changes on the microarray plate surface in response to a drug/small molecule binding to cell receptors. The results are measured in triplicate and results averaged. Baseline reference is subtracted from the data.


Antibody-antigen interactions example: Endpoint analysis of GRP78 protein binding to anti-GRP78 antibody.

Figure 2.  (a) Schematic of a guided-mode resonance sensor. The optical resonant modes are two different polarizations of light that are excited on the sensor surface and used to probe the binding reactions.

Figure 2. Endpoint analysis of GRP78 protein binding to anti-GRP78 antibody. Individual quadruplicate values were plotted at each concentration of GRP78 and the resultant scatter plot fitted to a nonlinear 4-PL curve fit.


Biomarker detection in cell-culture supernatant samples: correlation with Western Blot

Figure 2. (b) Binding events occurring at the sensor surface produce resonance-peak shifts that can be tracked in real time.

Figure 3. Measured relative protein levels for Fibronectin that are normalized with respect to the media control. Measurements were performed using the RSI detection system and compared with Western blot with good agreement. Known cancer cell lines Caov-3, SK-OV-3, OVCAR-3, TOV-21G, and TOV-112D were used. [Taken from Simon Kaja, Jill D. Hilgenberg, Julie L. Clark, Anna A. Shah, Debra Wawro, Shelby Zimmerman, Robert Magnusson, and Peter Koulen, "Detection of novel biomarkers for ovarian cancer with an optical nanotechnology detection system enabling label-free diagnostics," Journal of Biomedical Optics, vol. 17, no. 8, pp. 081412-1–081412-8, August 2012.]